Metal resistance sequences and transgenic plants

Overview

Information

Number
5965796
Application
08/878957
Filing date
06/19/1997
Issue date
10/12/1999
Inventor(s)
Richard Brian Meagher, Anne O. Summers, Clayton L. Rugh
US Classification
435/320.1; 435/419; 435/468; 435/69.1; 536/23.2; 536/23.7; 536/24.1; 800/278; 800/288; 800/295; 800/298
View at USPTO

Citations

UNIVERSITY OF GEORGIA, THE (1)
Edenspace Systems (1)
B & H Manufacturing (1)
CELLECTIS (1)

Abstract

Abstract

The present invention provides nucleic acid sequences encoding a metal ion resistance protein, which are expressible in plant cells. The metal resistance protein provides for the enzymatic reduction of metal ions including but not limited to divalent Cu, divalent mercury, trivalent gold, divalent cadmium, lead ions and monovalent silver ions. Transgenic plants which express these coding sequences exhibit increased resistance to metal ions in the environment as compared with plants which have not been so genetically modified. Transgenic plants with improved resistance to organometals including alkylmercury compounds, among others, are provided by the further inclusion of plant-expressible organometal lyase coding sequences, as specifically exemplified by the plant-expressible merB coding sequence. Furthermore, these transgenic plants which have been genetically modified to express the metal resistance coding sequences of the present invention can participate in the bioremediation of metal contamination via the enzymatic reduction of metal ions. Transgenic plants resistant to organometals can further mediate remediation of organic metal compounds, for example, alkylmetal compounds including but not limited to methyl mercury, methyl lead compounds, methyl cadmium and methyl arsenic compounds, in the environment by causing the freeing of mercuric or other metal ions and the reduction of the ionic mercury or other metal ions to the less toxic elemental mercury or other metals.

Assignees with Similar Patents

Monsanto (6)
NA (6)
Verenium (5)
Ceres (5)
University of Massachusetts (4)

Referenced by

UNIVERSITY OF GEORGIA, THE (3)
The Trustees of The University Of Pennsylvania (2)
University of South Carolina (2)

Tech Analysis

Class Subclass Type Reference Factor Citation Factor Strength Grant time Early grant (%)
435 320.1 Emerging Sustaining Average Medium Average 25.6
435 419 Emerging Sustaining Seminal Medium Average 25.2
435 468 Emerging Average Seminal Medium Average 44.5
435 69.1 Emerging Sustaining Average Medium Average 25.2
536 23.2 Emerging Sustaining Average Medium Average 31.5
536 23.7 Emerging Average Seminal Medium Average 28.4
536 24.1 Emerging Sustaining Average Medium Rapid 23.8
800 278 Emerging Average Seminal Medium Average 35.7
800 288 Emerging Average Seminal Medium Average 31.0
800 295 Emerging Average Seminal Medium Average 38.8
800 298 Emerging Average Seminal Medium Average 32.5