Therapeutic grade thrombin produced by chromatography
Overview
Citations
- Pfizer (4)
-
| Number |
Title |
Issue Date |
| 4696812 |
Thrombin preparations |
1987/09/29 |
| 4965203 |
Purified thrombin preparations |
1990/10/23 |
| 5151355 |
Process for the production of high purity thrombin |
1992/09/29 |
| 5397704 |
Ultra-pure thrombin preparation |
1995/03/14 |
- Yoshitomi Pharmaceutical Industries, (1)
-
| Number |
Title |
Issue Date |
| 5130244 |
Stable aqueous thrombin solution |
1992/07/14 |
- Medtronic (1)
-
| Number |
Title |
Issue Date |
| 5229172 |
Modification of polymeric surface by graft polymerization |
1993/07/20 |
- Baxter International (1)
-
| Number |
Title |
Issue Date |
| 5354682 |
Viral-safe purified human thrombin |
1994/10/11 |
- basf beauty care solutions france sas (1)
-
| Number |
Title |
Issue Date |
| 5331092 |
Process of preparation of collagen containing in major proportion insoluble collagen and collagen having high mechanical resistance and thermal stability obtained thereby |
1994/07/19 |
- Mochida Pharmaceutical Co. (1)
-
| Number |
Title |
Issue Date |
| 5149540 |
Thrombin composition for oral administration |
1992/09/22 |
- Leuven Research & Development V.Z.W. (1)
-
| Number |
Title |
Issue Date |
| 4202872 |
Determination of heparin in blood plasma |
1980/05/13 |
- The Green Cross (1)
-
| Number |
Title |
Issue Date |
| 4923815 |
Process for heat treating thrombin |
1990/05/08 |
- Omrix Biopharmaceuticals (1)
-
| Number |
Title |
Issue Date |
| 5143838 |
Method of producing thrombin from factor II using calcium ions for the conversion on an anion exchanger |
1992/09/01 |
- NA (1)
-
| Number |
Title |
Issue Date |
| 4083957 |
Process for the alteration of the sex-ratio of mammals |
1978/04/11 |
- SEROLOGICALS ROYALTTY (1)
-
| Number |
Title |
Issue Date |
| 4380511 |
Purification of bovine thrombin |
1983/04/19 |
- New York Blood Center (1)
-
| Number |
Title |
Issue Date |
| 4540573 |
Undenatured virus-free biologically active protein derivatives |
1985/09/10 |
Abstract
Abstract
A process for the large-scale production of therapeutic grade thrombin of excellent viral safety and storage-stability is carried out by purification of viricide treated crude thrombin by ion-exchange chromatography on a single column using a sulfalkyl-activated polysaccharide, particularly a non-compressible composite medium of sulfoalkyl-activated dextran and silica particles, as the ion exchange medium and using increasing concentrations of phosphate buffer for elution. After recovery of thrombin in the final eluate, the phosphate buffer is exchanged for a stabilizing formulation buffer, and the stabilized thrombin is subjected to viral filtration and optional dry heat treatment for further viral inactivation. The final product has high specific activity and is obtained in good yield.
Assignees with Similar Patents
Referenced by
- Asahi Kasei Medical Co. (3)
-
| Number |
Title |
Issue Date |
| 6274090 |
Apparatus and method of preparation of stable, long term thrombin from plasma and thrombin formed thereby |
2001/08/14 |
| 6472162 |
Method for preparing thrombin for use in a biological glue |
2002/10/29 |
| 7056722 |
Apparatus and method of preparation of stable, long term thrombin from plasma and thrombin formed thereby |
2006/06/06 |
- Omrix Biopharmaceuticals (3)
-
| Number |
Title |
Issue Date |
| 11008560 |
Method for purifying and quantifying thrombin and its degradation polypeptides |
2021/05/18 |
| 11149263 |
Method for purifying and quantifying thrombin and its degradation polypeptides |
2021/10/19 |
| 12351843 |
Method for purifying and quantifying thrombin and its degradation polypeptides |
2025/07/08 |
- Bristol-Myers Squibb (2)
-
| Number |
Title |
Issue Date |
| 5795571 |
Composition containing autologous thrombin blood fraction for use in a medical procedure |
1998/08/18 |
| 5795780 |
Method of use of autologous thrombin blood fraction in a cell culture with keratinocytes |
1998/08/18 |
- Grifols, (2)
-
| Number |
Title |
Issue Date |
| 7442308 |
Process for removing viruses in fibrinogen solutions and fibrinogen obtained by said process |
2008/10/28 |
| 9376674 |
Process to prepare a stable thrombin composition |
2016/06/28 |
- Arteriocyte Medical Systems (1)
-
| Number |
Title |
Issue Date |
| 7413652 |
Method for the production of a blood component composition |
2008/08/19 |
- Mitsubishi Pharma (1)
-
| Number |
Title |
Issue Date |
| 5945103 |
Process for producing thrombin |
1999/08/31 |
- CSL Behring (1)
-
| Number |
Title |
Issue Date |
| 5723123 |
Process for the production of a virus-free concentrate of thrombin |
1998/03/03 |
- Drying Technology (1)
-
| Number |
Title |
Issue Date |
| 5702715 |
Reinforced biological sealants |
1997/12/30 |
- Angiotech International (1)
-
| Number |
Title |
Issue Date |
| 5981254 |
Process for producing thrombin from plasma |
1999/11/09 |
Tech Analysis
| Class |
Subclass |
Type |
Reference Factor |
Citation Factor |
Strength |
Grant time |
Early grant (%) |
|
435
|
214
|
Stable |
Sustaining |
Seminal |
Medium |
Rapid |
14.5 |
|
435
|
815
|
Declining |
Sustaining |
Average |
Medium |
Rapid |
23.4 |